MACS Matchmaker
Documentation
This manual is intended to guide both novice and experienced users through the principles, implementation, and optimization of Focal Molography (FM) assays. It includes explanations of underlying theory, best practices in chip functionalization and assay design, as well as troubleshooting tips and analysis strategies.
Use this landing page to jump to focused documentation areas. Each section collects the material relevant to that aspect of the MACS Matchmaker platform.
Use the search field to find words and formulas anywhere in the manual; results link to the relevant page.
Getting Started
Your first measurement on the MACS Matchmaker — from powering on to viewing results.
Biomolecular Interaction Analysis (BIA)
Advanced background on binding theory, kinetics, affinity, and model concepts for users who want deeper scientific context.
MACS Matchmaker Concept
Summaries of the MACS Matchmaker platform, reader concept, and high-level workflows.
Backfilling and NSB suppression
What backfilling is, how to choose and tune the backfill probe, and how to verify an affinity-matched chip for measurements in complex media.
How to immobilize my ligand(s)?
Practical guide to choosing an immobilization strategy: amine coupling, capture, click chemistry, and the orientation, density, and reference-channel decisions that shape kinetic data quality.
Instrument Operation
Guidelines for daily operation.
Molographic Chips
Guides for preparing chips, including handling, functionalization steps, and quality controls.
Experiment Design
Guidelines for setting up common experiment types on the MACS Matchmaker platform.
Measurement Workflow
Measurement workflow steps, best practices, and troubleshooting tips for running assays on the MACS Matchmaker.
Data Analysis
Guidelines for data processing and analysis using the MACS Matchmaker software.
Safety & Compliance
Regulatory requirements, safety procedures, and compliance checklists for operating the platform.
Maintenance & Troubleshooting
Maintenance guidelines, operational issues, troubleshooting and quick recovery steps.
Appendix
Glossary, abbreviations, and supplemental reference material.
Tools
Selected tools and utilities to use alongside the MACS Matchmaker workflow.